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KMID : 0616120180490040322
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Korean Journal of Pharmacognosy
2018 Volume.49 No. 4 p.322 ~ p.327
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Anti-oxidative Effect of Salvia miltiorrhiza Bunge in Caenorhabditis elegans
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Kim Yeong-Jee
Kim Jun-Hyeong
Noh Yun-Jeong
Kim Su-JIn
Hwang In-Hyun
Kim Dae-Keun
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Abstract
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Methanol extract of Salvia miltiorrhiza Bunge (Labiatae) root was investigated to research the anti-oxidative activity, by using a Caenorhabditis elegans model system. The methanol extract of this plant showed significant DPPH radical scavenging and superoxide quenching activities. Ethyl acetate soluble fraction of the methanol extract that showed the most potent DPPH radical scavenging and superoxide quenching activities. The ethyl acetate fraction was tested on its activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance in C. elegans. Furthermore, in order to see if regulation of stress-response genes is responsible for the increased stress tolerance of the ethyl acetate fraction treated C. elegans, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of S. miltiorrhiza root increased the catalase and SOD activities in a dose?dependent manner in C. elegans. Besides, the ethyl acetate fraction-treated CF1553 worms showed higher SOD-3::GFP intensity than the non-treated ones.
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KEYWORD
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Salvia miltiorrhiza Bunge, Caenorhabditis elegans, Anti-oxidative effect, Catalase, SOD
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